Journal: Bioactive Materials

Article Title: Mesenchymal stem cell activity across a graded scaffold-hydrogel composite biomaterial for tendon-to-bone enthesis repair

doi: 10.1016/j.bioactmat.2025.07.017

Figure Lengend Snippet: Histological evaluation of triphasic scaffold structure and matrix remodeling. FFPE sections of hMSC-seeded triphasic scaffolds (n = 2) at both Day 1 and Day 21 were stained with either trichrome, alizarin red, or alcian blue staining. Representative images are provided of (A) a scan of a full scaffold (trichrome only), (B) the osseous region, (C) the tendinous region, and (D) the interfacial region.

Article Snippet: Samples were stained by one of four staining procedures: hematoxylin/eosin (H&E, SL200 & SL201, StatLab), alizarin red (Cat# 2003999, EMD Millipore Corp.), trichrome (Cat# ab150686, Abcam), or alcian blue (pH 2.5, Cat# KTABP2.5, StatLab) with staining procedures followed according to manufacturer recommendation.

Techniques: Staining

Journal: Gastroenterology

Article Title: Kr?ppel-like Factor 5 is Important for Maintenance of Crypt Architecture and Barrier Function in Mouse Intestine

doi: 10.1053/j.gastro.2011.06.086

Figure Lengend Snippet: Loss of a restricted differentiation zone and changes in epithelial cell lineage with deletion of Klf5. Paraffin-embedded colon sections from adult control and Klf5 mutant mice were stained with various epithelial markers to identify changes in differentiation. (A) Immunohistochemical staining with the colonic differentiation marker, carbonic anhydrase-I. (B) Quantitative real time polymerase chain reaction (qRT-PCR) to measure relative mRNA expression of Cdx1 and Cdx2 in colon tissues from control and mutant mice (*P < 0.001, **P = 0.004, n=3). (C) Alcian blue/Periodic acid-Schiff (PAS) staining to identify goblet cells and immunohistochemical staining of chromogranin A as a marker for enteroendocrine cells.

Article Snippet: Alcian blue/PAS staining of paraffin-embedded sections to identify goblet cells was carried out using the Alcian Blue and PAS Staining Kit (Biocare Medical, Concord, CA) per manufacturer's instructions.

Techniques: Control, Mutagenesis, Staining, Immunohistochemical staining, Marker, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing

Journal: Stem Cells International

Article Title: Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mouse Model of Parkinson's Disease: A New Biological Therapy for Parkinson's Disease

doi: 10.1155/2020/4636397

Figure Lengend Snippet: Phenotypic characterization and differentiation of hUC-MSCs. (a) Flow cytometry showing that isolated cultured hUC-MSCs highly expressed CD105, CD73, and CD90 and were negative for hematopoietic markers CD19, CD34, and CD45 and for the lymphocyte surface marker HLA-DR. (b) (A) Gross morphology of hUC-MSCs under light microscopy, (B) oil-red-O staining for the adipogenic differentiation of hUC-MSCs after 3 weeks, (C) endogenous alkaline phosphatase staining for the osteogenic differentiation of hUC-MSCs, and (D) Alcian blue staining of hUC-MSCs after 3 weeks for chondrogenic differentiation (scale bar = 100 μ m).

Article Snippet: The sections were analyzed for chondrogenic differentiation using a commercially available Alcian blue kit (IHC World, Woodstock).

Techniques: Flow Cytometry, Isolation, Cell Culture, Marker, Light Microscopy, Staining

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Cold Atmospheric Plasma Jet Promotes Wound Healing Through CK2-Coordinated PI3K/AKT and MAPK Signaling Pathways

doi: 10.1016/j.mcpro.2025.100962

Figure Lengend Snippet: Histological staining of extracellular matrix proteins in CAPJ-treated wound tissues. Rat skin cutaneous wound was treated with CAPJ for 60s. CAPJ-treated wound tissue section (CAPJ), with parallel untreated control (Ctrl), were prepared on day-3, -7, and -14, and subjected for histochemical staining by using Masson’s Trichrome Stain kit for collagen staining ( top panel, in dark blue color), Elastic Stain kit for elastin staining ( middle panel, in black color), and Alcian Blue Stain kit for hyaluronic acid (HA) staining ( button panel, in light-blue color). The red arrows were the extensively stained area for collagen and elastic fibers formation as well as the conjugates of HA and proteoglycans. Right panel correspond to high-magnification images of each cropped area on low-magnification image. Scale bars in low- and high-magnification images are 20 μm and 5 μm, respectively. CAPJ, cold atmospheric plasma jet.

Article Snippet: Tissue section (5 μm thick) was prepared in a standard fashion and observed in detail using Masson’s Trichrome Stain kit (StatLab, #KTMTR), Elastic Stain Kit (Modified Verhoeff's, ScyTek, ETS-1-IFU) and Alcian Blue (pH 2.5) Stain Kit (ScyTek, AFR-1-IFU).

Techniques: Staining, Control, Clinical Proteomics